Publications and pre-prints

UFMylation regulates translational homeostasis and cell cycle progression

 

Igor A. Gak, Djordje Vasiljevic, Thomas Zerjatke, Lu Yu, Mario Brosch, Theodoros I. Roumeliotis, Cindy Horenburg, Nancy Klemm, Gábor Bakos, Alexander Herrmann, Jochen Hampe, Ingmar Glauche, Jyoti S. Choudhary and Jörg Mansfeld. UFMylation regulates translational homeostasis and cell cycle progression.

BioRxiv, doi: https://doi.org/10.1101/2020.02.03.931196

Efficient and gentle delivery of molecules into cells with different elasticity via Progressive Mechanoporation.

 

Alena Uvizl Ruchi GoswamiShanil Durgeshkumar GandhiMartina AugsburgFrank BuchholzJochen GuckJörg MansfeldSalvatore Girardo (2021). Efficient and gentle delivery of molecules into cells with different elasticity via Progressive Mechanoporation.

Lab Chip 2021 Jun 15;21(12):2437-2452. doi: 10.1039/d0lc01224f.

Redox potential defines functional states of adult hippocampal stem cells.

 

Vijay S Adusumilli, Tara L Walker, Rupert W Overall, Gesa M Klatt, Salma A Zeidan, Tim J Fischer, Sara Zocher, Alex M Sykes, Susanne Reinhardt, Andreas Dahl, Dilyana G Kirova, Jörg Mansfeld, Annette E Rünker, Gerd Kempermann (2019). Redox potential defines functional states of adult hippocampal stem cells.

Cell Stem Cell 2021 Feb 4;28(2):300-314.e6. doi: 10.1016/j.stem.2020.10.019.

Dimerization regulates the human APC/C-associated ubiquitin-conjugating enzyme UBE2S.

 

Anna K. L. Liess*, Alena Kucerova*, Kristian Schweimer, Dörte Schlesinger, Olexandr Dybkov, Henning Urlaub, Jörg Mansfeld# and Sonja Lorenz# (2020). Dimerization regulates the human APC/C-associated ubiquitin-conjugating enzyme UBE2S.

Science Signaling (13), 654:eaba8208

                                                                                                                                          *shared first authorship

                                                                                                                                          #corresponding authors

Degron-tagged reporters probe membrane topology and enable the specific labelling of membrane-wrapped structures.

 

Katharina B Beer, Gholamreza Fazeli, Kristyna Judasova, Linda Irmisch, Jona Causemann, Jörg Mansfeld, Ann M Wehman (2019). Degron-tagged reporters probe membrane topology and enable the specific labelling of membrane-wrapped structures.

Nature Communications 10:3490

Autoinhibition Mechanism of the Ubiquitin-Conjugating Enzyme UBE2S by Autoubiquitination.

 

Anna K.L. Liess*, Alena Kucerova*, Kristian Schweimer, Lu Yu, Theodoros I. Roumeliotis, Mathias Diebold, Olexandr Dybkov, Christoph Sotriffer, Henning Urlaub, Jyoti S. Choudhary, Jörg Mansfeld#, Sonja Lorenz#

(2019). Autoinhibition Mechanism of the Ubiquitin-Conjugating Enzyme UBE2S by Autoubiquitination

Structure. 2019 Jun 20,  doi: 10.1016/j.str.2019.05.008

                                                                                                                                          *shared first authorship

                                                                                                                                          #corresponding authors

Conditional control of fluorescent protein degradation by an auxin-dependent nanobody.

 

Daniel K., Icha, J.,  Horenburg, C., Müller D., Norden C., and Mansfeld J. (2018). Conditional control of fluorescent protein degradation by an auxin-dependent nanobody

Nature Communications 9:3297.                                                                                                 

Distinct Levels of Reactive Oxygen Species Coordinate Metabolic Activity with Beta-cell Mass

 

Alfar E.A*.,  Kirova D*., Konantz J., Birke S., Mansfeld J#., and Ninov N#. (2017). Distinct Levels of Reactive Oxygen Species Coordinate Metabolic Activity with Beta-cell Mass Plasticity.

Scientific Reports 7:3994                                                                                               *shared first authorship

                                                                                                                                          #corresponding authors

PLK1 Activation in Late G2 Sets Up Commitment to Mitosis.

 

Gheghiani L.,  Loew D., Lombard B., Mansfeld J., and  Gavet O. (2017). PLK1 Activation in Late G2 Sets Up Commitment to Mitosis.

Cell Reports 19:2060-73

DNA damage during S-phase mediates the proliferation-quiescence decision in the subsequent G1 via p21 expression.

 

Barr AR., Cooper S., Heldt FS., Butera F., Stoy H., Mansfeld J., Novák B., Bakal C. (2017). DNA damage during S-phase mediates the proliferation-quiescence decision in the subsequent G1 via p21 expression.

Nature  Communications  8:14728.

A human interactome in three quantitative dimensions organized by stoichiometries and abundances.

 

Hein, MY., Hubner NC., Poser I., Cox J., Nagaraj N., Toyoda Y., Gak I., Weisswange i., Mansfeld J., Buchholz F., Hyman AA., Mann M. (2015). A human interactome in three quantitative dimensions organized by stoichiometries and abundances.

Cell 163(3):712-23.

Real-time deformability cytometry: on-the-fly cell mechanical phenotyping.

 

Otto, O., Rosendahl, P., Mietke, A., Golfier, S., Herold, C., Klaue, D., Girardo, S., Pagliara, S., Ekpenyong, A., Jacobi, A.,Wobus M., Toepfer N., Keyser UF., Mansfeld J., Fischer-Friedrich E., Guck, J. (2015). Real-time deformability cytometry: on-the-fly cell mechanical phenotyping.

Nature Methods 12(3):199-202.

The ABBA Motif Binds APC/C Activators and Is Shared by APC/C Substrates and Regulators.

 

Di Fiore, B., Davey, N. E., Hagting, A., Izawa, D., Mansfeld, J., Gibson, T. J., and Pines, J. (2015). The ABBA Motif Binds APC/C Activators and Is Shared by APC/C Substrates and Regulators.

Developmental Cell 32:358–372.

APC15 drives the turnover of MCC-CDC20 to make the spindle assembly checkpoint responsive to kinetochore attachment.

 

Mansfeld, J., Collin, P., Collins, M. O., Choudhary, J. S., and Pines, J. (2011). APC15 drives the turnover of MCC-CDC20 to make the spindle assembly checkpoint responsive to kinetochore attachment.

Nature Cell Biology 13:1234–1243.

Pom121 links two essential subcomplexes of the nuclear pore complex core to the membrane.

 

Mitchell, J. M., Mansfeld, J., Capitanio, J., Kutay, U., and Wozniak, R. W. (2010). Pom121 links two essential subcomplexes of the nuclear pore complex core to the membrane.

Journal of J Cell Biology 191:505–521.

UBE2S elongates ubiquitin chains on APC/C substrates to promote mitotic exit.

 

Garnett, M. J*., Mansfeld, J*., Godwin, C., Matsusaka, T., Wu, J., Russell, P., Pines, J., and Venkitaraman, A. R. (2009). UBE2S elongates ubiquitin chains on APC/C substrates to promote mitotic exit.

Nature Cell Biology 11:1363–1369.                                                                               *shared first authorship

The actin multigene family of Paramecium tetraurelia.

 

Sehring, I. M., Mansfeld, J., Reiner, C., Wagner, E., Plattner, H., and Kissmehl, R. (2007). The actin multigene family of Paramecium tetraurelia.

BMC Genomics 8:82.

A broad spectrum of actin paralogs in Paramecium tetraurelia cells display differential localization and function.

 

Sehring, I. M., Reiner, C., Mansfeld, J., Plattner, H., and Kissmehl, R. (2007). A broad spectrum of actin paralogs in Paramecium tetraurelia cells display differential localization and function.

Journal of Cell Science 120: 177–190.

The conserved transmembrane nucleoporin NDC1 is required for nuclear pore complex assembly in vertebrate cells.

 

Mansfeld, J*., Güttinger, *S., Hawryluk-Gara, L. A., Panté, N., Mall, M., Galy, V., Haselmann, U., Mühlhäusser, P., Wozniak, R. W., Mattaj, I. W., et al. (2006). The conserved transmembrane nucleoporin NDC1 is required for nuclear pore complex assembly in vertebrate cells.

Molecular Cell 22:93–103.                                                                                              *shared first authorship

A multigene family encoding R-SNAREs in the ciliate Paramecium tetraurelia.

 

Schilde, C., Wassmer, T., Mansfeld, J., Plattner, H., and Kissmehl, R. (2006). A multigene family encoding R-SNAREs in the ciliate Paramecium tetraurelia.

Traffic 7:440–455.