The Mansfeld Lab
Publications and pre-prints
UFMylation regulates translational homeostasis and cell cycle progression
Igor A. Gak*, Djordje Vasiljevic*, Thomas Zerjatke, Lu Yu, Mario Brosch, Theodoros I. Roumeliotis, Cindy Horenburg, Nancy Klemm, Gábor Bakos, Alexander Herrmann, Jochen Hampe, Ingmar Glauche, Jyoti S. Choudhary and Jörg Mansfeld.
BioRxiv, doi: 10.1101/2020.02.03.931196
Super-resolution microscopy reveals focal organization of ER-associated Y-complexes in mitosis.
Rojapriyadharshini Gandhimathi, Dorothea Pinotsi, Mario Köhler, Jörg Mansfeld, Caroline Ashiono, Tatjana Kleele, Sumit Pawar and Ulrike Kutay
EMBO Reports (2023)24:e56766: https://doi.org/10.15252/embr.202356766
A ROS-dependent mechanism promotes CDK2 phosphorylation to drive progression through S phase.
Developmental Cell: https://doi.org/10.1016/j.devcel.2022.06.008
Efficient and gentle delivery of molecules into cells with different elasticity via Progressive Mechanoporation.
Alena Uvizl* , Ruchi Goswami*, Shanil Durgeshkumar Gandhi, Martina Augsburg, Frank Buchholz, Jochen Guck, Jörg Mansfeld# and Salvatore Girardo# (2021).
Lab Chip 2021 Jun 15;21(12):2437-2452. doi: 10.1039/d0lc01224f.
#corresponding authors
Redox potential defines functional states of adult hippocampal stem cells.
Vijay S Adusumilli, Tara L Walker, Rupert W Overall, Gesa M Klatt, Salma A Zeidan, Tim J Fischer, Sara Zocher, Alex M Sykes, Susanne Reinhardt, Andreas Dahl, Dilyana G Kirova, Jörg Mansfeld, Annette E Rünker, Gerd Kempermann (2019).
Cell Stem Cell 2021 Feb 4;28(2):300-314.e6. doi: 10.1016/j.stem.2020.10.019.
Dimerization regulates the human APC/C-associated ubiquitin-conjugating enzyme UBE2S.
Anna K. L. Liess*, Alena Kucerova*, Kristian Schweimer, Dörte Schlesinger, Olexandr Dybkov, Henning Urlaub, Jörg Mansfeld# and Sonja Lorenz# (2020).
Science Signaling (13), 654:eaba8208. doi: 10.1126/scisignal.aba8208
#corresponding authors
Degron-tagged reporters probe membrane topology and enable the specific labelling of membrane-wrapped structures.
Katharina B Beer, Gholamreza Fazeli, Kristyna Judasova, Linda Irmisch, Jona Causemann, Jörg Mansfeld, Ann M Wehman (2019).
Nature Communications 10:3490. doi: 10.1038/s41467-019-11442-z
Autoinhibition Mechanism of the Ubiquitin-Conjugating Enzyme UBE2S by Autoubiquitination.
Anna K.L. Liess*, Alena Kucerova*, Kristian Schweimer, Lu Yu, Theodoros I. Roumeliotis, Mathias Diebold, Olexandr Dybkov, Christoph Sotriffer, Henning Urlaub, Jyoti S. Choudhary, Jörg Mansfeld#, Sonja Lorenz#
(2019).
Structure. 2019 Jun 20, doi: 10.1016/j.str.2019.05.008
#corresponding authors
An E2-ubiquitin thioester-driven approach to identify substrates modified with ubiquitin and ubiquitin-like molecules.
Bakos, G., Yu l., Gak IA., Roumeliotis, TI., Liakopoulos D., Choudhary JS. and Mansfeld J. (2018).
Nature Communications 9:4776. doi: 10.1038/s41467-018-07251-5
Conditional control of fluorescent protein degradation by an auxin-dependent nanobody.
Daniel K*., Icha, J*., Horenburg, C., Müller D., Norden C#., and Mansfeld J#. (2018).
Nature Communications 9:3297. doi: 10.1038/s41467-018-05855-5
#corresponding authors
Constitutive regulation of mitochondrial morphology by Aurora A kinase depends on a predicted cryptic targeting sequence at the N-terminus.
Grant R., Abdelbaki A., Bertoldi A., Gavilan MP., Mansfeld J., Glover DM. and Lindon C. (2018).
Quantitative Cell Cycle Analysis Based on an Endogenous All-in-One Reporter for Cell Tracking and Classification.
Distinct Levels of Reactive Oxygen Species Coordinate Metabolic Activity with Beta-cell Mass
Alfar E.A*., Kirova D*., Konantz J., Birke S., Mansfeld J#., and Ninov N#. (2017).
Scientific Reports 7:3994. doi: 10.1038/s41598-017-03873-9
#corresponding authors
PLK1 Activation in Late G2 Sets Up Commitment to Mitosis.
Gheghiani L., Loew D., Lombard B., Mansfeld J., and Gavet O. (2017).
Cell Reports 19:2060-73. doi: 10.1016/j.celrep.2017.05.031
DNA damage during S-phase mediates the proliferation-quiescence decision in the subsequent G1 via p21 expression.
Barr AR., Cooper S., Heldt FS., Butera F., Stoy H., Mansfeld J., Novák B., Bakal C. (2017).
Nature Communications 8:14728. doi: 10.1038/ncomms14728
A human interactome in three quantitative dimensions organized by stoichiometries and abundances.
Hein, MY., Hubner NC., Poser I., Cox J., Nagaraj N., Toyoda Y., Gak I., Weisswange i., Mansfeld J., Buchholz F., Hyman AA., Mann M. (2015).
Cell 163(3):712-23. doi: 10.1016/j.cell.2015.09.053
Real-time deformability cytometry: on-the-fly cell mechanical phenotyping.
Otto, O., Rosendahl, P., Mietke, A., Golfier, S., Herold, C., Klaue, D., Girardo, S., Pagliara, S., Ekpenyong, A., Jacobi, A.,Wobus M., Toepfer N., Keyser UF., Mansfeld J., Fischer-Friedrich E., Guck, J. (2015).
Nature Methods 12(3):199-202. doi:10.1038/nmeth.3281
The ABBA Motif Binds APC/C Activators and Is Shared by APC/C Substrates and Regulators.
Di Fiore, B., Davey, N. E., Hagting, A., Izawa, D., Mansfeld, J., Gibson, T. J., and Pines, J. (2015).
Developmental Cell 32:358–372. doi: 10.1016/j.devcel.2015.01.003
APC15 drives the turnover of MCC-CDC20 to make the spindle assembly checkpoint responsive to kinetochore attachment.
Mansfeld, J., Collin, P., Collins, M. O., Choudhary, J. S., and Pines, J. (2011).
Nature Cell Biology 13:1234–1243. doi: 10.1038/ncb2347
Pom121 links two essential subcomplexes of the nuclear pore complex core to the membrane.
Mitchell, J. M., Mansfeld, J., Capitanio, J., Kutay, U., and Wozniak, R. W. (2010).
Journal of J Cell Biology 191:505–521. doi: 10.1083/jcb.201007098
UBE2S elongates ubiquitin chains on APC/C substrates to promote mitotic exit.
Garnett, M. J*., Mansfeld, J*., Godwin, C., Matsusaka, T., Wu, J., Russell, P., Pines, J., and Venkitaraman, A. R. (2009).
Nature Cell Biology 11:1363–1369. doi: 10.1038/ncb1983
The actin multigene family of Paramecium tetraurelia.
Sehring, I. M., Mansfeld, J., Reiner, C., Wagner, E., Plattner, H., and Kissmehl, R. (2007).
BMC Genomics 8:82. doi: 10.1186/1471-2164-8-82
A broad spectrum of actin paralogs in Paramecium tetraurelia cells display differential localization and function.
Sehring, I. M., Reiner, C., Mansfeld, J., Plattner, H., and Kissmehl, R. (2007).
Journal of Cell Science 120: 177–190. doi: 10.1242/jcs.03313
The conserved transmembrane nucleoporin NDC1 is required for nuclear pore complex assembly in vertebrate cells.
Mansfeld, J*., Güttinger, *S., Hawryluk-Gara, L. A., Panté, N., Mall, M., Galy, V., Haselmann, U., Mühlhäusser, P., Wozniak, R. W., Mattaj, I. W., Kutay U#. and Antonin W#. (2006).
Molecular Cell 22:93–103. doi: 10.1016/j.molcel.2006.02.015
#corresponding authors
A multigene family encoding R-SNAREs in the ciliate Paramecium tetraurelia.
Schilde, C., Wassmer, T., Mansfeld, J., Plattner, H., and Kissmehl, R. (2006).
Traffic 7:440–455. doi: 10.1111/j.1600-0854.2006.00397.x