Finally, after three rounds of revisions Katrin's paper on an auxin-dependent nanobody to target GFP-tagged proteins for degradation has been published in Nature Communications. If you ever wanted to get rid of your favorite GFP-tagged protein try our mAID-nanobody! Together with Jaroslav Icha from Caren Norden's lab and help from Cindy and Doris we show that mAID-nanobody-dependent degradation works in human cell lines and in zebrafish. Thereby, we demonstrate for the first time that the auxin system can also be applied to a vertebrate model organism. Have a look here to find out how it works!
In July, Joerg and Alena attended the 43rdFEBS Congress "Biochemistry forever" Prague 2018. For Alena it was exciting time going for the first big conference and presenting her results at the poster session. Jörg talked about Katrin's paper on the "Conditional control of fluorescent protein degradation by an auxin-dependent nanobody" in the biotechnology session.
In July, Kristyna went to Spain to find out what is new in redox biology at the GRC on Thiol-Based Redox and Signaling. She presented a poster with findings about Redox oscillations during the cell cycle and got useful feedback. Moreover, she met very nice people who were willing to share free time on the beach, which was valuable time for informal discussion, relaxing and getting the strength for more talks.
Have a look at the latest paper we contributed to: Rhys Grant from Catherine Lindon's Lab at the University of Cambridge used a endogenous Aurora A-Venus knock' in we generated to uncover a role for Aurora A in the regulation of mitochondrial morphology. Read the story in the June 2018 edition of Open Biology.
This year, the Mansfeld lab and five external guests got together to discuss science at our Lab Retreat in the beautiful surrounding of Spreewald. We spent four sunny days discussing ubiquitin, redox and APC/C projects ongoing in the lab and enjoyed rowing on the river while trying out local specialties....
Have a first glance Katrin's and Jaroslav's story that is now available as a preprint on bioRxiv. In a collaboration with the Norden Lab we show how GFP-tagged proteins can be targeted for degradation by an auxin-dependent nanobody in human cells and in zebrafish.
We are seeking highly motivated and creative colleagues to explore how reversible redox modifications control cell cycle decisions. We employ cell biological and biochemical techniques with an emphasis on single cell imaging and protein interactions. Several ambitious projects are available and can be tailored to individual interests and expertise.
Applicants should have a strong expertise in redox biology ideally involving cell biological techniques and/or protein biochemistry. Additional experience with programing languages such as Python, Matlab, Mathematica or R is beneficial. English language skills are essential; knowledge of German is not required. The position(s) can start immediately or upon mutual agreement. Salary will be according to the German TVL scale. In case of equivalent qualifications, disabled applicants will be preferentially considered. PhD applicants will have the possibility to additionally apply to the Dresden International PhD Programm (DIPP, http://www.dresd...
Time flies! The Mansfeld lab is operating since January 2013 and so we have just turned 5!
This alone was a great occasion to gather together and celebrate. However, we also made toasts for Gabor's thesis submission, him securing the DIGS-BB Wrap-up Grant and Alena becoming the DIGS-BB fellow. In the meantime we threw darts and potted some billiard balls. What a night!
Congratulations to Alena for becoming the DIGS-BB Fellow! Here's what she has to say about her project and the award:
"The small molecule APC/C inhibitors I will develop during my PhD might be beneficial for cancer therapy in the future, especially in the light that indirect inhibition of the APC/C is already used for cancer treatment. Thanks to DIGS-BB Fellow Award I can move our ambitious project ahead and since it is my first own funding, it will also help fostering my future scientific career."
Big day for Gabor today as he submitted his PhD Thesis entitled "Target identification of ubiquitin- and ubiquitin-like-modified substrates of specific E2-E3 pairs by E2~dID". Looking forward to Gabor's defence next year!