Alena's first paper from a beautiful collaboration with Anna Liess from Sonja Lorenz' group in Würzburg just got published in Structure! Combining structural biology, biochemistry and cell biology our labs together reveal how ubiquitination of a conserved lysine residue close to the active site of the E2 ubiquitin-conjugating enzyme UBE2S regulates its activity and thereby the ability to generate K11-linked ubiquitin chains during mitosis.
Have a look here to read the full story and below to see a happy Alena - Well deserved!!
Have a look here for Dilyana's collaboration with Gerd Kempermann's lab at the CRTD/DZNE in Dresden, who show that the redox potential defines functional states of adult hippocampal stem cells. Great work by Vijay, Tara, Rupert and everybody else involved!
Ann Wehman's lab from the Virchow Center of the University of Würzburg developed a wonderful degron-based approach to probe membrane topology by light microscopy in C. elegans. Kristyna from our lab showed that this approach can also be applied to human cells and to auxin-mediated degradation by monitoring the stability of mAID-tagged lamin A, a component of the nuclear lamina. In the movie below you can see that nuclear mAID-tagged lamin A is only degraded during mitosis after nuclear envelope breakdown in cells where TIR1- the ubiquitin ligase adapter responsible for mAID-dependent degradation is otherwise sequestered to the cytoplasm. Hence, only when the nuclear and cytoplasmic compartments mix in mitosis can mAID-lamin A be degraded and thus precisely marks the loss of nuclear envelope permeability. Have a look at the pre-print on bioRxiv to see how this approach works in detail and how it can help studying your preferred organelle.
Another PhD. from Mansfelds: Dr. Gábor Bakos! He defended his work on a method for identification of ubiquitin- and ubiquitin-like-modified substrates of specific E2-E3 enzyme pairs by E2~dID and also successfully passed the Rigorosum. At the end of the day he left with a big smile and a Magna cum laude. Well done, Gábor! ....though his skills opening champagne bottles certainly require another PhD..have look at slideshow to find out more :-)
Have a first look at Gabor's pre-print ot the bioRxiv describing a new technique, E2~dID, that is ideally suited to identify the substrates specific E2/E3 ubiquitin enzymes in your favorite experiential model.
Igor successfully defended his PhD thesis and became a well deserved "Dr. Gak". He did well during the Rigorosum and the presentation about his work on "Targeted loss-of-function screen for cell cycle regulating E2 conjugating enzymes identifies UFC1 as a regulator of G1 phase progression" and received a Magna cum laude. Congrats Igor!!
Finally, after three rounds of revisions Katrin's paper on an auxin-dependent nanobody to target GFP-tagged proteins for degradation has been published in Nature Communications. If you ever wanted to get rid of your favorite GFP-tagged protein try our mAID-nanobody! Together with Jaroslav Icha from Caren Norden's lab and help from Cindy and Doris we show that mAID-nanobody-dependent degradation works in human cell lines and in zebrafish. Thereby, we demonstrate for the first time that the auxin system can also be applied to a vertebrate model organism. Have a look here to find out how it works!
In July, Kristyna went to Spain to find out what is new in redox biology at the GRC on Thiol-Based Redox and Signaling. She presented a poster with findings about Redox oscillations during the cell cycle and got useful feedback. Moreover, she met very nice people who were willing to share free time on the beach, which was valuable time for informal discussion, relaxing and getting the strength for more talks.
Have a look at the latest paper we contributed to: Rhys Grant from Catherine Lindon's Lab at the University of Cambridge used a endogenous Aurora A-Venus knock' in we generated to uncover a role for Aurora A in the regulation of mitochondrial morphology. Read the story in the June 2018 edition of Open Biology.